The sample to be tested is inoculated into one or more vials which are inserted into the bd bactec fluorescent. In a previous work, we showed that for patients admitted to the emergency department of our hospital, the combination of bactec lytic anaerobic and bactec aerobic vials was more efficient than bactec. There was no significant difference in the recovery of individual groups of organisms with the two systems, with the exception of. Bactec plus blood culture bottles were preincu bated at. Performance of a new combination of blood culture vials in. Apparently, the revised software in the bactec 9240 analyzer version 3. The instrument monitors the fluorescence of the vial sensor which increases as co 2 is produced or oxygen is utilized. Biogrouping, mic, and antimicrobial susceptibility profiles of isolates jundishapur j microbiol. Pdf direct comparison of the bactec 9240 and bactalert 3d. Pdf use of the bactec 9240 system with mycosisicf blood. A suspension of each specimen was made in 5 ml of normal saline and adjusted to a 0. Principle when microorganisms are present in culture vials, they metabolize nutrients in the culture medium, releasing carbon dioxide into the medium.
The full text of this article is available as a pdf 207k. Group 1, children with vascular catheterassociated bloodstream infection. Comparison of four methods for rapid identification of. Comparison of bactec 9240 and difco esp blood culture. A world of difference in blood culture page 3 072014 figure 2 fluorescent technology and sophisticated algorithms. Use of the bactec 9240 system with mycosisicf blood culture bottles for detection of fungemia a recent study by horvath et al. Controlled clinical comparison of isolator and bactec 9240. In the bactec 9050, the wilcoxon median time to detection. Positive bottles were removed from the system and subcultured. The overall recovery of organisms and time to detection with the bactec 9050 and bactec 9240 systems were compared in a multicenter evaluation.
Truepositive and falsepositive results evaluation of bactec. Bactec 9240 a fluorogenic, automated, and noninvasive blood culture system from becton dickinson. The percent recovery, difference between predicate and modified bottles with 95% twosided bootstrap confidence intervals stratified by. Dickinson diagnostic systems and incubated in a bactec 9240 continuously monitored blood culture system. The bactec 9240 was compared with the bactec nr 660 for the detection of organisms and bacteremic. A dye in the sensor at the bottom of the vial reacts with co2.
Prospective study of the clinical performance of three bactec. Bactec 9240 alarm triggered by plasmodium falciparum. Recall, though, that our isolates were all incubated for a total of 12 days. Prospective study of the clinical performance of three. Bactec 9240 91209050 bactec fluorescent series microorganisms, if present in the blood samples, metabolize nutrients in the bactec culture vial and release co2 into the medium or utilize the oxygen in the medium. The bactec 9050 exhibited a statistically significant difference in time to deteciion between the new and predicate devices. Bd bactec 9120 9240 instruments can also be easily combined with bd bactec fx modules via bd epicenter tm. Block scientific is an acclaimed distributor in the united states, offering a fine line of lab equipment for sale including chemistry analyzers, hematology analyzers, centrifuges, histology equipment, reagents, consumables, and more. Newsletter best practices in blood culture collection. Use of a pair of blood culture bottles for sterility testing. The diagnosis of bloodstream infection requires the optimum combination of media in an automated blood culture system for maximum recovery of pathogens with the earliest time to detection. Extra vial sequence barcode labels can be used to replace damaged or unreadable labels on culture vials.
Blood volume in bactec plus f culture bottles sampled using. Multicenter comparison of bactec 9050 and bactec 9240. Experience accumulated with the bactec 9240 instrument has demonstrated that the system enables detection of b. This functionality, called bit for builtintest, automatically monitors each station every ten. Mar 16, 2002 these keywords were added by machine and not by the authors. Blood cultures i clinical microbiology and infection.
We also thank walter mika for assistance with phlebotomy and john a. Comparison of the two blood culture systems, bactec 9240 and. Bactec fluorescent series instruments bactec 9050, bactec 9240, bactec fx, and bactec fx40 using software versions listed below. The sensitivity and time to detection of brucella melitensis by the bactec 9240 and the isolator blood culture systems were compared in a prospective volumecontrolled study. Use of a pair of blood culture bottles for sterility. Changes in colour or turbidity of storage media were evaluated daily at the corneal bank. The detection of microorganisms in a patients blood has diagnostic and prognostic importance. Multicenter clinical evaluation of a continuous monitoring blood culture system using fluorescentsensor technology bactec 9240. Use of incubation time to detection in bactec 9240 to. Pdf a direct comparison of two automated blood culture systems was conducted to compare their ability to detect candida growth.
Evaluation of bactec 9240 blood culture system by using. Mar 16, 2002 bactec 9240 alarm triggered by plasmodium falciparum bactec 9240 alarm triggered by plasmodium falciparum richalet, g brenierpinchart, m bosseray, a croize, j pelloux, h. The systems evaluated were the bactec 9240 bactec and bactalert 3d bact. Pmc free article nolte fs, williams jm, jerris rc, morello ja, leitch cd, matushek s, schwabe ld, dorigan f, kocka fe. Comparison of the two blood culture systems, bactec 9240. This process is experimental and the keywords may be updated as the learning algorithm improves.
However, using a single peds plusf bottle the few episodes of anaerobic bacteraemia that occur in children are likely to be missed. Each organism was tested in triplicate at 0, 8, 24, 36, and 48 h and was incubated at both room temperature rt and 35 degrees c. Comparison of bactec 9240 peds plus medium and isolator. Bd bactec instrumented blood culture systems from bd. Of 463 positive blood culture sets, 956 bottles were positive, of which the instrument detected 92. In conclusion, the bactec 9240 used with highvolume aerobicf medium is equivalent to the bactec 660 used with high volume nr26 medium for the detection of microorganisms and bacteremic episodes. Becton dickinson bactec fx microbiological culture analyzer. The bactec 9240 blood culture system becton dickinson diagnostic instrument systems, sparks.
Controlled clinical laboratory comparison of two supplemented aerobic and. The aims of the present study were to investigate the isolation of salmonella spp. In the first phase of the study, a total of 4,383 compliant aerobic plus aerobicf blood culture sets were processed. Evaluation of bactec 9240 blood culture system by using high.
The peds plusf bottle was used because in our laboratory the quantity of. The bactec 9240 blood culture system becton dickinson diagnostic instrument systems, sparks, md. Direct comparison of the bactec 9240 and bactalert 3d. The 9240 system required 23 s less technologist time per bottle to operate during the 5day protocol. Bd bactec 9240 system accommodates 240 test bottles. This article cites 6 articles, 6 of which can be accessed free at. Bactec 9240 and difco esp aerobic and anaerobic vials were inoculated with human blood and were seeded with organism growth from 18 species, including obligate aerobic, anaerobic, and facultative anaerobic organisms. In the late 1950s, the company was sold to an investor who took it in the direction of seeking government contracts to study beta radiation and develop sensitive. Recipients were screened postgraft for infectious signs. Use of bactec 9240 blood culture system for detection of.
Ziegler r, johnscher i, martus p, lenhardt d, just hm. A recovery comparison of the new device versus the predicate device results in a mcnemar pvalue of 1. Each medium was inoculated in a pair of bactec plus aerobicf and bactec lytic10 anaerobicf blood bottles and placed in a bactec 9240 incubator for 14 days at 37c and in a sabouraud broth at 20c. The bd bactec automated blood culture system utilizes fluorescent technology in detecting the growth of organisms in the blood culture bottles. The full text of this article is available as a pdf 104k.
Sep 14, 2019 bactec 9240 blood culture system, bd bactec 9240. Jun 29, 2005 four ml of dilutions containing less than 1 to greater than 10 3 colony. A total of 28 clinical isolates of escherichia coli, staphylococcus aureus, candida albicans, and candida glabrata were studied. Kolbert, truepositive and falsepositive results evaluation of bactec 9240 instrument and sequencing of the 16s rrna gene. For information or assistance, call toll free 1800638. Synovial fluid specimens aspirated from patients with arthritis were inoculated into an aerobic peds plus blood culture bottle and monitored by the bactec 9240 instrument for 4 weeks. Rapid diagnosis of brucella bacteremia by using the bactec 9240. Three groups of febrile pediatric patients with blood cultures growing cons were identified. Becton dickinson bactec 9240 blood culture system for sale. Bactec automated blood culture system learn microbiology. Bd bactec 9240 9120 system users manual 62 8085863200903 figure 61 maintenance log. William johnston and colleagues from purdue university founded a company that specialized in developing instruments to detect very low levels of carbon14, which were marketed for use in air quality testing. Comparison of four methods for rapid identification of staphylococcus aureus directly from bactec 9240 blood culture system ns ozen 1, d ogunc 1, d mutlu 1, g ongut 1, bo baysan 1, f gunseren 2 1 department of clinical microbiology, akdeniz university medical faculty, antalya, turkey 2 department of clinical microbiology and infectious diseases, akdeniz university medical faculty, antalya, turkey.
Compared with the signal system, the bactec 9240 offers markedly more rapid and sensitive detection of bacteraemia, together with a lower rate of nonsignificant isolates. Specialized media that accommodate smallvolume samples to help optimally detect common pediatric and nonpediatric organisms. Experience of changing between signal and bactec 9240 blood. Truepositive and falsepositive results evaluation of. Fx, bactec 9240 and bactec 9050 fluorescentseries blood culture instruments. Comparison of bactec 9240 peds plus medium and isolator 1. Bactec 9 0 5 0 system users manual figure 3 main s t a t u s screen 1. Use of incubation time to detection in bactec 9240 to distin. Bactec plus blood culture bottles were preincubated at 35c or at room temperature before entry into the bactec 9240 instrument to determine the influence of preincubation temperature and time. Of 76 positive bottles undetected by the instrument, 68 were preincubated at 35c and eight at. An evaluation was undertaken to determine the utility of the bactec peds plusf bottle and the bactec 9240 instrument becton dickinson diagnostic instrument systems, sparks, md. Nov 01, 2016 in this study, we compared the performance of bactec plus aerobicf and bactec plus anaerobicf reference set, both containing resins, with bactec plus aerobicf and bactec lytic10 anaerobicf test set, the latter with saponin. Capacity 240 test bottles bactec 9240 system, 120 test bottles bactec 9120 system, 50 test vials, bactec 9050 system dimensions inquire analysis time 10 mins.
Bd bactec 9120 9240 instruments can also be easily combined with bd bactec fx modules via bd epicenter. The bactec 9240 and bactec fx both did not have a statistically significant difference in time to detection between the new and predicate devices. Comparison of the effect of delayed entry into 2 different blood culture systems bactec 9240 and bactalert 3d on culture positivity. The convenient location of the touch screen and barcode scanner also improves ergonomics. Bd bactec tm 9000 series bd bactec tm instruments product size description code 445475 1 bd bactec tm blood culture system bd bactec tm 9240 features include continuous monitoring of culture specimens, noninvasive design, unique fluorescent sensor technology, reliability and robustness, and simple operation. In the present study, we found false positives in 57 2. Comparison of bactec 9240 and difco esp blood culture systems.
Multicenter comparison of bactec 9050 and bactec 9240 blood. Bactec plus blood culture bottles were preincubated at 35 degrees c or at room temperature before entry into the bactec 9240. Blood sample aliquots, obtained from children with suspected brucellosis, were inoculated into a bactec 9240 peds plus bottle and into an isolator 1. Bactec 924091209050 bactec fluorescent series microorganisms, if present in the blood samples, metabolize nutrients in the bactec culture vial and release co 2 into the medium or utilize the oxygen in the medium. The purpose of this investigation was to evaluate the performance of the bactec 9240 and bactalert 3d blood culture systems in the detection of candida spp. In conclusion, selection of the bactec 9240 together with the pluslytic media combination was associated with increased positivity rates, significantly more septicemia drg codings and a substantial increase in monies reimbursed to the system. The bactec 9050 system is well suited for laboratories that process relatively few blood culture bottles and should offer significant advantages over the manual or. A total of 1,072 synovial fluid cultures were processed, and 15 0. New and used medical equipment, hospital equipment.
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